Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is a tumoural cytokine released from melanoma
Identifieur interne : 000F35 ( Main/Exploration ); précédent : 000F34; suivant : 000F36Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is a tumoural cytokine released from melanoma
Auteurs : Ambra A. Grolla [Italie] ; Simone Torretta [Italie] ; Ilaria Gnemmi [Italie] ; Angela Amoruso [Italie] ; Giuseppe Orsomando [Italie] ; Marco Gatti [Italie] ; Antonio Caldarelli [Italie] ; Dmitry Lim [Italie] ; Lorenza Penengo [Italie, Suisse] ; Sandra Brunelleschi [Italie] ; Armando A. Genazzani [Italie] ; Cristina Travelli [Italie]Source :
- Pigment Cell & Melanoma Research [ 1755-1471 ] ; 2015-11.
Abstract
High plasma levels of nicotinamide phosphoribosyltransferase (NAMPT), traditionally considered an intracellular enzyme with a key role in NAD synthesis, have been reported in several oncological, inflammatory and metabolic diseases. We now show that eNAMPT can be actively released by melanoma cells in vitro. We analysed the mechanisms of its release, and we found both classical and non‐classical pathway involvement. eNAMPT released by melanoma cells, in our hands, has paracrine and autocrine effects: it activates MAPK, AKT and NF‐κB pathways and increases colony formation in anchorage‐independent conditions. eNAMPT also induces M1 polarization in human monocytes. Last, we demonstrate, for the first time in any cancer type, that eNAMPT levels in plasma of tumour‐bearing mice increase and that this increase can be reconducted to the tumour itself. This provides an important cue on previous observations that eNAMPT is increased in patients with cancer. Moreover, silencing NAMPT in melanoma cells leads to a reduction in the tumour growth rate. Our findings extend the basis to consider eNAMPT as a cytokine involved in tumour progression.
Url:
DOI: 10.1111/pcmr.12420
Affiliations:
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Genazzani</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Pharmaceutical Sciences and Department of Medical Sciences, Università del Piemonte Orientale, Novara</wicri:regionArea><wicri:noRegion>Novara</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Italie</country></affiliation></author><author><name sortKey="Travelli, Cristina" sort="Travelli, Cristina" uniqKey="Travelli C" first="Cristina" last="Travelli">Cristina Travelli</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Pharmaceutical Sciences and Department of Medical Sciences, Università del Piemonte Orientale, Novara</wicri:regionArea><wicri:noRegion>Novara</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Pigment Cell & Melanoma Research</title><title level="j" type="alt">PIGMENT CELL AND MELANOMA RESEARCH</title><idno type="ISSN">1755-1471</idno><idno type="eISSN">1755-148X</idno><imprint><biblScope unit="vol">28</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="718">718</biblScope><biblScope unit="page" to="729">729</biblScope><biblScope unit="page-count">13</biblScope><date type="published" when="2015-11">2015-11</date></imprint><idno type="ISSN">1755-1471</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1755-1471</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">High plasma levels of nicotinamide phosphoribosyltransferase (NAMPT), traditionally considered an intracellular enzyme with a key role in NAD synthesis, have been reported in several oncological, inflammatory and metabolic diseases. We now show that eNAMPT can be actively released by melanoma cells in vitro. We analysed the mechanisms of its release, and we found both classical and non‐classical pathway involvement. eNAMPT released by melanoma cells, in our hands, has paracrine and autocrine effects: it activates MAPK, AKT and NF‐κB pathways and increases colony formation in anchorage‐independent conditions. eNAMPT also induces M1 polarization in human monocytes. Last, we demonstrate, for the first time in any cancer type, that eNAMPT levels in plasma of tumour‐bearing mice increase and that this increase can be reconducted to the tumour itself. This provides an important cue on previous observations that eNAMPT is increased in patients with cancer. Moreover, silencing NAMPT in melanoma cells leads to a reduction in the tumour growth rate. Our findings extend the basis to consider eNAMPT as a cytokine involved in tumour progression.</div></front></TEI><affiliations><list><country><li>Italie</li><li>Suisse</li></country><region><li>Canton de Zurich</li></region><settlement><li>Zurich</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Grolla, Ambra A" sort="Grolla, Ambra A" uniqKey="Grolla A" first="Ambra A." last="Grolla">Ambra A. Grolla</name></noRegion><name sortKey="Amoruso, Angela" sort="Amoruso, Angela" uniqKey="Amoruso A" first="Angela" last="Amoruso">Angela Amoruso</name><name sortKey="Brunelleschi, Sandra" sort="Brunelleschi, Sandra" uniqKey="Brunelleschi S" first="Sandra" last="Brunelleschi">Sandra Brunelleschi</name><name sortKey="Caldarelli, Antonio" sort="Caldarelli, Antonio" uniqKey="Caldarelli A" first="Antonio" last="Caldarelli">Antonio Caldarelli</name><name sortKey="Gatti, Marco" sort="Gatti, Marco" uniqKey="Gatti M" first="Marco" last="Gatti">Marco Gatti</name><name sortKey="Genazzani, Armando A" sort="Genazzani, Armando A" uniqKey="Genazzani A" first="Armando A." last="Genazzani">Armando A. Genazzani</name><name sortKey="Genazzani, Armando A" sort="Genazzani, Armando A" uniqKey="Genazzani A" first="Armando A." last="Genazzani">Armando A. Genazzani</name><name sortKey="Gnemmi, Ilaria" sort="Gnemmi, Ilaria" uniqKey="Gnemmi I" first="Ilaria" last="Gnemmi">Ilaria Gnemmi</name><name sortKey="Lim, Dmitry" sort="Lim, Dmitry" uniqKey="Lim D" first="Dmitry" last="Lim">Dmitry Lim</name><name sortKey="Orsomando, Giuseppe" sort="Orsomando, Giuseppe" uniqKey="Orsomando G" first="Giuseppe" last="Orsomando">Giuseppe Orsomando</name><name sortKey="Penengo, Lorenza" sort="Penengo, Lorenza" uniqKey="Penengo L" first="Lorenza" last="Penengo">Lorenza Penengo</name><name sortKey="Torretta, Simone" sort="Torretta, Simone" uniqKey="Torretta S" first="Simone" last="Torretta">Simone Torretta</name><name sortKey="Travelli, Cristina" sort="Travelli, Cristina" uniqKey="Travelli C" first="Cristina" last="Travelli">Cristina Travelli</name></country><country name="Suisse"><region name="Canton de Zurich"><name sortKey="Penengo, Lorenza" sort="Penengo, Lorenza" uniqKey="Penengo L" first="Lorenza" last="Penengo">Lorenza Penengo</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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